The main duty of the tonoplast is to separate the other contents of from the cytoplasm. The vacuole is surrounded by a membrane named the tonoplast. Most of the time, it contains the threads of cytoplasm, running through the vacuole. The size of the plant vacuole is enough for occupying a very large area of the volume of the cell. It is large in size, situated in the center of the cell and exists mostly in the mature plant cells. You will find only one vacuole in the plant cell. Difference between Plant Vacuole and Animal Vacuole
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